Calmodulin yeast complementation

A Deep Mutational Scan of human Calmodulin using functional complementation in yeast.


Created June 29, 2018
Last updated Aug. 8, 2019
Published June 29, 2018
Member of urn:mavedb:00000001

Score sets






Although we now routinely sequence human genomes, we can confidently identify only a fraction of the sequence variants that have a functional impact. Here, we developed a deep mutational scanning framework that produces exhaustive maps for human missense variants by combining random codon mutagenesis and multiplexed functional variation assays with computational imputation and refinement. We applied this framework to four proteins corresponding to six human genes: UBE2I (encoding SUMO E2 conjugase), SUMO1 (small ubiquitin-like modifier), TPK1 (thiamin pyrophosphokinase), and CALM1/2/3 (three genes encoding the protein calmodulin). The resulting maps recapitulate known protein features and confidently identify pathogenic variation. Assays potentially amenable to deep mutational scanning are already available for 57% of human disease genes, suggesting that DMS could ultimately map functional variation for all human disease genes.



A Deep Mutational Scan of Calmodulin (CALM1) using functional complementation in yeast was performed using DMS-TileSeq and a machine-learning method was used to impute the effects of missing variants and refine measurements of lower confidence. See Weile et al. 2017 for details.


  • Weile J, et al. A framework for exhaustively mapping functional missense variants. Mol. Syst. Biol. 2017; 13:957. PMID: 29269382




Name: CALM1

Type: Protein coding

Organism: Homo sapiens

Reference genome: hg38

Reference assembly: Other/Synthetic


External identifiers

DOI: No associated DOIs

Raw reads:

Score sets

  • Human Calmodulin DMS-TileSeq

    A Deep Mutational Scan of human Calmodulin using functional complementation in yeast via DMS-TileSeq.

  • Human Calmodulin imputed and refined

    A machine-learning imputed and refined Deep Mutational Scan of human Calmodulin using functional complementation in yeast.