HMGCR yeast complementation

A deep mutational scan of human HMG-CoA reductase (HMGCR) based on a functional complementation assay in yeast.

urn:mavedb:00000035-a

Created July 10, 2019
Last updated July 15, 2019
Published July 15, 2019
Member of urn:mavedb:00000035

Score sets

urn:mavedb:00000035-a-1

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urn:mavedb:00000035-a-2

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urn:mavedb:00000035-a-3

Abstract

Untitled

Coronary heart disease (CHD) is a leading cause of death worldwide. The risk of CHD can be reduced by LDL-cholesterol-lowering medications called statins that potently inhibit HMG-CoA reductase (HMGCR), the enzyme that catalyzes the rate-limiting reaction of the mevalonate pathway. Proactive maps of human HMGCR may be able to model patient response to statins and identify the statin with the greatest likelihood of efficacy.

Method

Untitled

The yeast-based assay of HMGCR is a functional complementation assay that assesses the ability of human HMGCR to rescue the growth of an hmg1 hmg2 double deletion yeast strain in glucose media (Basson et al. 1988). We followed the TileSeq approach (Weile & Sun et al, MSB, 2017) to produce variant effect maps of HMGCR in glucose media without a statin, and media supplemented with atorvastatin or rosuvastatin.

References

No associated publications.

Contributors

Keywords

No keywords are associated with this entry.

Targets

Name: HMGCR

Type: Protein coding

Organism: Homo sapiens

Reference genome: hg38

Reference assembly: Other/Synthetic

Reference sequence: ATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGAGGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTGGGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGAATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACAGGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCAGGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGAGCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTATGTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTAAAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAAGTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCATCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGAGAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTTGGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGGTCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCAGATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAAGGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGAAGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACAGGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCAGATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACAGGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGA

External identifiers

DOI: No associated DOIs

Raw reads: No associated raw reads

Score sets

  • urn:mavedb:00000035-a-1

    HMGCR rosuvastatin imputed and refined

    A deep mutational scan of HMG-CoA reductase (HMGCR) based on a functional complementation assay in yeast via DMS-TIleSeq in rosuvastatin media.

  • urn:mavedb:00000035-a-2

    HMGCR no statin imputed and refined

    A deep mutational scan of human HMG-CoA reductase (HMGCR) based on a functional complementation assay in yeast via DMS-TileSeq in glucose media with no statin.

  • urn:mavedb:00000035-a-3

    HMGCR atorvastatin imputed and refined

    A deep mutational scan of human HMG-CoA reductase (HMGCR) based on a functional complementation assay in yeast via DMS-TileSeq in atorvastatin media.